Cellular protein expression profiles within tissues are key to understanding disease pathology, and their spatial organisation determines cellular function. To precisely define molecular phenotypes in the spatial context of tissue, there is a need …
Background: Multimerization is a key process in prion-like disorders such as Alzheimer’s disease (AD), since it is a requirement for self-templating tau and beta-amyloid amyloidogenesis. AT8-immunohistochemistry for hyperphosphorylated tau is …
The pathological hallmark of amyotrophic lateral sclerosis (ALS) is the presence of cytoplasmic inclusions, containing C terminal fragments of the protein TDP‐43. Here, we tested the hypothesis that highly sensitive mass spectrometry with parallel …
Here, we reconstruct 3D fibre orientations from 2D microscopy images in the BigMac dataset. This will facilitate future determination of the ultra-high resolution microscopy connectome, with applications in basic neuroanatomy and tractography design.
Here, we describe an end-to-end pipeline for the extraction of a histological metric from IHC stains to quantify a microstructural feature. We compare the pipeline's reproducibility and robustness to histology artefacts, relative to manual MRI-histology analyses.
Here, we describe an end-to-end pipeline for the extraction of a histological metric from IHC stains to quantify a microstructural feature. We compare the pipeline's reproducibility and robustness to histology artefacts, relative to manual MRI-histology analyses.
Degeneration of the primary motor cortex is a defining feature of amyotrophic lateral sclerosis (ALS), which is associated with the accumulation of microscopic protein aggregates in neurons and glia. However, little is known about the quantitative …
In this proof of principle experiment, we demonstrate that MALDI-MSI combined with classical LCM LC-MS/MS has the potential to spatially resolve metabolic signatures in human brain at cellular resolution. Abstract & Talk given - The Oxford Metabolic Health Symposium 2020, 30th June – 3rd July 2020.
Diffusion-weighted steady-state free precession (DW-SSFP) is an SNR-efficient diffusion imaging method. The improved SNR and resolution available at ultra-high field has motivated its use at 7T. However, these data tend to have severe B1 …
LC-MS based mass spectrometry imaging of proteins has the potential to provide novel insights into the proteome of tissues. Abstract - Presented at the Oxford Metabolic Health Symposium 2020, 30th June – 3rd July 2020.