The Digital Brain Bank, an open access platform for post-mortem datasets
Post mortem MRI provides the opportunity to acquire high resolution datasets to investigate neuroanatomy, and validate the origins of image contrast through microscopy comparisons. We introduce the Digital Brain Bank (open.win.ox.ac.uk/DigitalBrainBank), a data release platform providing open access to curated, multimodal post-mortem neuroimaging datasets.
Tau-proximity ligation assay reveals extensive previously undetected pathology prior to neurofibrillary tangles in preclinical Alzheimer’s disease
Background:
Multimerization is a key process in prion-like disorders such as Alzheimer’s disease (AD), since it is a requirement for …
Multimerization is a key process in prion-like disorders such as Alzheimer’s disease (AD), since it is a requirement for …
Detection and quantification of novel C-terminal TDP-43 fragments in ALS-TDP
The pathological hallmark of amyotrophic lateral sclerosis (ALS) is the presence of cytoplasmic inclusions, containing C terminal …
Quantitative patterns of motor cortex proteinopathy across ALS genotypes
Degeneration of the primary motor cortex is a defining feature of amyotrophic lateral sclerosis (ALS), which is associated with the …
Use of multi-flip angle measurements to account for transmit inhomogeneity and non-Gaussian diffusion in DW-SSFP
Diffusion-weighted steady-state free precession (DW-SSFP) is an SNR-efficient diffusion imaging method. The improved SNR and resolution …
Rapid intraoperative molecular genetic classification of gliomas using Raman spectroscopy.
Developing an approach to use Raman spectroscopy to classify gliomas based on genetic subtypes.
Development of a Sensitive, Scalable Method for Spatial, Cell-Type-Resolved Proteomics of the Human Brain
While nearly comprehensive proteome coverage can be achieved from bulk tissue or cultured cells, the data usually lacks spatial resolution. As a result, tissue based proteomics averages protein abundance across multiple cell types and/or localizations. With proteomics platforms lacking sensitivity and throughput to undertake deep single-cell proteome studies in order to resolve spatial or cell type dependent protein expression gradients within tissue, proteome analysis has been combined with sorting techniques to enrich for certain cell populations. However, the spatial resolution and context is lost after cell sorting. Here, we report an optimized method for the proteomic analysis of neurons isolated from post-mortem human brain by laser capture microdissection (LCM).
Single-copy expression of an amyotrophic lateral sclerosis-linked TDP-43 mutation (M337V) in BAC transgenic mice leads to altered stress granule dynamics and progressive motor dysfunction
Modelling an amyotrophic lateral sclerosis BAC transgenic mouse haboring a TDP43(M337V) mutation.
Is vascular endothelial growth factor a useful biomarker in giant cell arteritis?
To assess the performance of circulating vascular endothelial growth factor (VEGF) levels as a tool for diagnosing giant cell arteritis (GCA) in a cohort of patients referred for assessment of suspected GCA.
